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KMID : 0903519880310030267
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1988 Volume.31 No. 3 p.267 ~ p.273
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Yeast Cloning Vectors and their Application to the Development of Starch - fermenting Yeast
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Abstract
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Transformed, hybrid strains of the yeast Saccharomyces capable of simultaneous secretion of both glucoamylase and α-amylase have been produced. These strains can carry out direct, one-step assimilation of starch with conversion efficiency greater than 93% during a 5 day growth period. One of the transformants converts 92.8% of available starch into reducing sugars in only 2 days. Glucoamylase secretion by these strains results from expression of one or more chromosomal STA genes derived from Saccharomyces diastaticus. The strains were transformed by a plasmid(pMS12) containing mouse salivary α-amylase cDNA in an expression vector containing yeast alcohol dehydrogenase promoter and a segment of yeast 2μ plasmid. The major starch hydrolysis product produced by crude amylases found in culture broths is glucose, indicating that α-amylase and glucoamylase act cooperatively.
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